高效毛细管电泳法测定三黄油擦剂中大黄素和大黄酚的含量

Content Determination of Emodin and Chrysophanol in Sanhuangyou Liniment by HPCE

目的:建立高效毛细管电泳(HPCE)法对三黄油擦剂中的有效成分大黄素和大黄酚进行含量测定。方法:采用石英毛细管柱(65 cm×55μm,有效长度50 cm),缓冲体系为10 mmol·L-1硼砂-30 mmol·L-1SDS-10%乙醇,pH值为9.8,分离电压30 kV,检测波长254 nm。结果:大黄素和大黄酚在20 min内得到很好的分离,分别在0.124～2.470 g·L-1、0.104～2.080 g·L-1线性关系良好,平均回收率分别为99.2%9、8.6%。结论:HPCE方法结果准确、简便快速、重现性好,可用于三黄油擦剂的质量控制。

Objective：To develop a method for the content determination of Emodin and Chrysophanol in Sanhuangyou liniment by HPCE. Methods：The fused silica capillary column（65 cm × 55 μm, effective length of 50 cm） was used. The electrolyte buffer system was composed of 10 mmol· L^-1 borax-30 mmol · L^-1 SDS- 10% ethanol with Ph of 9.8,separation voltage of 30 kV and detection wavelength of 254nm. Results ： Emodin and Chrysophanol were successfully separated within 20 min and the linear response range were 0. 124 -2. 470 and 0. 104 -2. 080 mg · mL^-1. The average recovery rates was 99.2% and 98.6% respectively. Conclusions： The method produces accurate results, which is simple, rapid and well reproducible, and could be used for the quantity control of Sanhuangyou liniment.