反相高效液相色谱法测定盾叶薯蓣中薯蓣皂苷的含量

RP-HPLC Method for the Content Determination of Dioscin in Dioscorea Zingiberensis Wright

目的:探讨盾叶薯蓣中薯蓣皂苷含量测定的方法。方法:采用反相高效液相色谱法。色谱柱为DIKMA C18色谱柱(250mm×4.6 mm,5μm),流动相为乙腈-水(5248),流速为1.0 mL·min-1,检测波长为203.0 nm,柱温为30℃。结果:薯蓣皂苷在0.40～4.01μg范围内线性关系良好,相关系数r=0.999 7(n=7)。平均回收率为101.65%,RSD为2.29%(n=6)。结论:该方法简便、准确、快速、灵敏、重现性好,可以作为盾叶薯蓣的质量控制方法。

Objective：To explore a method for the determination of the conten of dioscin in Dioscorea zingiberensis wright. Methods：RP- HPLC was used with DIKMA C18 chromatography eolumn （250 mm· 4.6 ram,5 μm） as the column and acetonitrilewater（ 52：48 ） as the mobile phase. The flow rate of the mobile phase was 1.0 mL · min^- l and the detection wavelength was 203.0 nm. The column temperature was 30 ℃. Results： The linear range for dioscin was 0.40 - 4.01 μg and the correlation coefficient was 0.999 7 （ n = 7 ）. The average recovery rate was 101.65 % and RSD was 2.29% （n = 6 ）. Conclusion：This method is simple, accurate, rapid, sensitive, reproducible,which can be regarded as the quality control method for Dioscorea zingiberensis wright.