熟地强筋合剂的定性鉴别和含量测定

Qualitative Identification and Content Determination of Shudi Qiangjin Mixture

目的:建立熟地强筋合剂的定性鉴别和含量测定方法。方法:采用薄层色谱法定性鉴别牡丹皮和甘草;采用高效液相色谱法测定山茱萸中马钱苷的含量。结果:薄层色谱法检出牡丹皮、甘草,阴性无干扰,专属性强;马钱苷在0.050 8～0.304 8μg范围内具有良好的线性关系(r=.0999 8),平均加样回收率98.2%,RSD=1.80%(n=6)。结论:本方法简便、准确、重复性好,可用于熟地强筋合剂的质量控制。

Objective ：To establish the methods for qualitative identification and content determination of Shudi Qiangjin Mixture. Meth- ods ：Moutan cortex and Radix glycyrrhlaze were identified by thin layer chromatography （TLC） and the content of Loganin in corni fructus was determined by high performance liquid chromatography（HPLC）. Results ： Moutan cortex and Radix glycyrrhlaze could be identified by TLC ,which presented negativity without interference and had strong specificity. The linear range of loganin was 0.050 8 - 0.304 8 μg（ r = 0. 999 8 ） and had good linear relation. The average recovery rate was 98.2%, RSD = 1.80% （n = 6）. Conclusion：This method is simple, accurate and repeatable, which can be used in the quality control of Shudi Qiangjin Mixture.