风湿骨痛凝胶贴膏中青藤碱体外释放及透皮吸收研究

Study on in Vitro Release and Transdermal Absorption of Sinomenine in Fengshi-Gutong Gel Plaster

目的:研究风湿骨痛凝胶贴膏中有效成分青藤碱的体外释放及透皮吸收特性。方法:采用Franz扩散池法,以微孔滤膜代替筛网,研究该制剂中青藤碱的体外释放;以小鼠腹皮为体外模型,研究该制剂中青藤碱的透皮吸收行为。结果:风湿骨痛凝胶贴膏中青藤碱12 h累积释放率可达97.76%,释放曲线符合Weibull方程,ln(1/(1-Q))=1.4012×lnt+0.2326,r=0.9875;12 h累积透皮吸收百分率为44.86%,透皮吸收过程符合Weibull方程,ln(1/(1-Q))=0.2118×lnt+0.0737,r=0.9874。结论:风湿骨痛凝胶贴膏中有效成分青藤碱体外释放及透皮吸收良好,具有较好的临床应用前景。

This study was aimed to evaluate the in vitro release and transdermal absorption of the sinomenine in Fengshi-Gutong gel plaster.Sinomenine was analyzed by HPLC.The in vitro release behavior of the preparation was investigated through the application of TP-5 intelligence penetration tester.And hairless abdominal skin was used in the transdermal absorption study.The results showed that the cumulative releasing percent of sinomenine in Fengshi-Gutong gel plaster was 97.76% within 12 h.And the release curve fitted the Weibull equation,ln（1 /（1-Q）） = 1.4012 × lnt ＋ 0.2326（r = 0.9875）.The cumulative transdermal absorption percent within 12 h was 44.86%.And the transdermal absorption process fitted the Weibull equation,ln（1/（1-Q）） = 0.2118 × lnt ＋0.0737（r = 0.9874）.It was concluded that results of in vitro release and transdermal absorption of sinomenine in Fengshi-Gutong gel plaster were favorable.Therefore,this preparation is worth further development.