针刺对去势雄兔干眼模型泪液分泌及泪腺上皮细胞相关蛋白Fas/FasL表达的影响

Acupuncture Castrated Rabbit Dry Eye Model Schirmer and Related proteins of the Lacrimal Gland Epithelial Cells Fas / FasL Expression

目的:探讨针刺对去势雄兔干眼动物模型泪液分泌及泪腺上皮细胞凋亡相关蛋白Fas、FasL表达的影响。方法:健康成年雄兔18只,随机分为正常组、干眼组和针刺组,每组6只。干眼组和针刺组行双侧睾丸及附睾切除制作干眼模型,2周后去势雄兔干眼模型形成并稳定后对针刺组进行局部针刺,各组兔分别于去势前和去势后1、2、3、4、5、6、7周检测泪流量,并对各组兔泪腺进行免疫组化研究。结果:针刺2周后针刺组泪液分泌增加,与干眼组相比差异有统计学意义(P<0.05)。7周后,对各组兔泪腺进行免疫组化,干眼组泪腺上皮细胞Fas、FasL表达明显高于针刺组,差异具有统计学意义(P<0.05),针刺组与正常组没有统计学差异(P>0.05)。结论:局部针刺能够抑制凋亡的发生,增加去势干眼雄兔泪液的分泌。

Objective：This article is to study the effect of acupuncture on tear secretion,and lacrimal gland epithelial pathological apoptosis-related proteins’ Fas/FasL of castrated male rabbits animal models with dry eye symptoms.Methods：Divide 18 healthy adult male rabbits randomly into normal group,dry eye group and acupuncture group（6 rabbits each group）.Do bilateral removal of the testis and epididymis on dry eye group and acupuncture group to produce dry eye models.After 2 weeks,do local acupuncture treatment on castrated male rabbits when the dry eye model formed and became stable.Test tears flow of each group of rabbits before castration and 1,2,3,4,5,6,7 weeks after castration.Take rabbit lacrimal glands of each group for immunohistochemical study.Results：After 2 weeks of acupuncture,tear secretion of acupuncture treatment group increased,and the result is statistically significant（P〈0.05）compared with the dry eye group.In the 7th week,rabbit lacrimal glands of each group for immunohistochemical,Fas/FasL of lacrimal gland epithelial cells in dry eye group is significantly higher than the acupuncture group,and the difference is statistically significant（P〈0.05）,acupuncture group and normal group was not statistically significant（P〉0.05）.Conclusion：Local acupuncture can inhibit the occurrence of apoptosis,increase castration dry eye the secretion of tears the rabbit.