九龙江下游水源水中新发病原微生物和抗生素抗性基因的定量PCR检测

Real-time PCR Detection and Quantification of Emerging Waterborne Pathogens (EWPs) and Antibiotic Resistance Genes (ARGs) in the Downstream Area of Jiulong River

新发水传病原微生物和抗生素抗性基因对于饮用水的生物安全性有重要意义.以7种典型的水传病原微生物和4种抗生素抗性基因作为检测指标,对闽西南地区的重要水源九龙江下游水源水进行了检测.从九龙江下游4个采样点和附近某水厂进出水采集水样,用0.22μm的滤膜过滤后,进行DNA提取及荧光定量PCR检测.结果表明,九龙江下游水源水中Salmonella enterica(Salmonella spp.)、Legionella pneumophila(L.pneumophila)和Pseudomonas aeruginosa(P.aeruginosa)等个别指标可达到103、104、105copies.mL-1的水平.有机物浓度可能对病原微生物和抗生素抗性基因的水平有较大影响.水厂常规工艺对于大多数病原微生物和抗生素抗性基因可以实现有效去除,但对Salmonella spp.的去除效果不理想.因此为保证饮用水安全,需要在清理污染源、提高处理工艺水平以及末端控制方面加强管理.

The emerging waterborne pathogens（EWPs） and antibiotic resistance genes（ARGs） are important for drinking water safety.The detection and quantification of 7 EWPs and 4 ARGs were carried out in Jiulong River,which is the main water source of southwestern Fujian Province.The water samples were collected from four sites of the Jiulong River downstream area and a drinking water treatment plant nearby.DNA was extracted and quantified by real-time（SYBR Green） PCR methods after the samples were filtered through 0.22 μm membranes.The results showed that the amount of Salmonella enterica（Salmonella spp.）,Legionella pneumophila（L.pneumophila） and Pseudomonas aeruginosa（P.aeruginosa） could reach up to 103,104and 105 copies·mL-1,respectively.The concentration of organic matter in water may affect the copy numbers significantly.The water plant could effectively remove most EWPs and ARGs except Salmonella spp..Therefore,more efforts should be made on water pollution source control,water treatment technology and point-of-use system to make sure the safety of drinking water.