黄柏中小檗碱的提取工艺优化及其对植物病原菌的抑制作用

Optimization of Extraction Technology for Berberine in Cortex Phellodendri and Its Inhibition Effect on Plant Pathogen

采用正交试验设计,研究了乙醇浓度、提取次数、温度和pH值4个因素对黄柏中小檗碱提取率的影响,通过分光光度比色法,测定小檗碱含量。采用药物平板稀释法测定提取液对不同菌种的抑制作用。结果表明,黄柏中小檗碱的最佳提取条件为:在40℃下,用pH 4.0的70%乙醇溶液提取2次。在最佳提取条件下,药液浓度15 mg/ml对黄瓜黑斑病菌的抑菌率达100%,药液浓度20 mg/ml对禾谷类作物病原菌的抑菌率达100%。此种提取工艺,黄柏中小檗碱产率高,且操作简单可行。

The extraction process for berberine in Cortex Phellodendri was optimized in ethanol concentration, extracting times, temperature and pH value by orthogonal design and the berberine content was detected by spectrophotometric method. The inhibition effect of extract on four species of plant pathogens were determined by plate diluting method. The optimal technology was extracting for 2 times with 70% ethanol under pH4.0 and 40℃. The antifungal rate against Alterharia alternate was 100% when the concentration of berberine was 15 mg/ml, while that was 100% against Fusarium culmorum when the concentration of berberine was 20 mg/ml. The method was simple and reliable with high output of berberine.