摘要
双向电泳技术是进行蛋白质组分分离和功能鉴定的重要步骤。提取和制备高质量的蛋白质是进行双向电泳的首要前提。杨树树皮组织中含有大量的干扰物质,影响了蛋白质的提取和双向电泳的分离效果。为建立一种适合杨树树皮蛋白质的双向电泳程序,本实验以107杨为材料,采用改良的三氯乙酸-丙酮沉淀法提取杨树树皮蛋白质,经双向电泳和考马斯亮蓝染色检测,得到了理想的双向电泳图谱,为杨树与病原互作的蛋白质组学研究奠定了基础。
Two - dimensional electrophoresis （2 - DE） is an important step for protein separation and function identification. Extracting and preparing high -quality protein is the first premise of 2 -DE. There is a large amount of interfering substances in poplar bark tissues, which influence the extraction of protein and separation effect of 2 - DE. In order to develop a 2 - DE procedure suitable for poplar bark protein, the poplar 107 was used as material. The improved method of trichloroacetic acid （TCA） -acetone was adopted to extract the proteins of poplar bark. After the two - dimensional electrophoresis and detection with Coomassie brilliant blue, the desirable gel maps were obtained. It layed the foundation for proteomics research of poplar and pathogen interaction.
出处
《山东农业科学》
2012年第7期121-123,共3页
Shandong Agricultural Sciences
基金
国家科技部基础性工作专项(2009FY210100)的部分研究内容
关键词
蛋白质组学
杨树树皮
双向电泳技术
Proteomics
Poplar bark
Two - dimensional electrophoresis
