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基因工程抗体亲和力成熟研究进展 被引量：1

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摘要 自1975年，Kohler创建杂交瘤技术并成功制备单克隆抗体以来，人们己研制出数以千计的单克隆抗体。单克隆抗体因其高度的靶向特异性已经成为科学研究，疾病诊断，药物靶向治疗等不可或缺的工具。山于杂交瘤技术制备单克隆抗体为鼠源性，在人体应用时易发生免疫排斥反应，难以有效激发人体免疫效应，而人源的基因工程抗体很好地解决了这一问题。借助DNA重组和蛋白质工程技术，人们可以获得人源单克隆抗体，
作者 于礼 梁米芳
机构地区 中国疾病预防控制中心病毒病预防控制所
出处 《中国医药生物技术》 CSCD 2012年第4期286-289,共4页 Chinese Medicinal Biotechnology
关键词 基因工程抗体 亲和力成熟 人源单克隆抗体 蛋白质工程技术 杂交瘤技术 药物靶向治疗 免疫排斥反应 靶向特异性
分类号 R392 [医药卫生—免疫学]
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参考文献31

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同被引文献45

  • 1汪保安,王琰.基因工程抗体的体外亲和力成熟[J].海军总医院学报,2003,16(4):222-226. 被引量：2
  • 2胡晓林,张春明.基因工程抗体亲和力成熟的策略[J].免疫学杂志,2006,22(6):702-704. 被引量：8
  • 3WEISSER N E, HALL J C. Applications of single-chain variable fragment antibodies in therapeutics and diagnostis. Biotechnology Advances, 2009,27 : 502-520.
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  • 5WEN S, ZHANG X, LIU Y, et al. Selection of a single chain variable fragment antibody against ivermectin from a phage displayed library. Journal of Agricultural and Food Chemistry, 2010, 58(9) :5387-5391.
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中国医药生物技术
2012年 第4期

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