30例自然流产患者绒毛滋养细胞凋亡及调控蛋白Bcl-2、Bax的表达研究

Study on apoptosis and expression of modulin Bcl-2, Bax in villus syncytiotrophoblast cells in 30 patients with spontaneous abortion

目的通过观察绒毛合体滋养细胞凋亡以及凋亡调控蛋白Bcl-2和Bax在自然流产患者中的表达,探讨细胞凋亡在自然流产的发病机制。方法采用原位末端标记法(TUNEL)和免疫组织化学法对30例自然流产患者绒毛合体滋养细胞中调亡指数及凋亡调控蛋白Bcl-2和Bax阳性表达率进行检测,并以30例人工终止早孕的健康妇女做对照(对照组),所有数据采用SPSS16.0 进行统计学分析。结果自然流产组绒毛滋养细胞中凋亡指数为(32.45±5.87)%,明显高于对照组的(19.38±4.16)%,差异有统计学意义(P<0.01)。Bax和Bcl-2在两组合体滋养细胞中的阳性率比较显示,自然流产组中Bax阳性率增高,Bcl-2阳性率下降,Bcl-2/Bax比值升高,差异均有统计学意义(P<0.01)。结论绒毛合体滋养细胞凋亡明显增加、Bax阳性率增高和Bcl-2阳性率下降在自然流产中产生重要作用。

Objective To observe the apoptosis and expression ofmodulin Bcl-2, Bax in villus syneytiotro- phoblast cells in patients with spontaneous abortion, and to investigate the pathogenesis of apoptosis in spontaneous abortion. Methods TdT-mediated dUTP nick end labeling （TUNEL） and immunohistochemistry were respectively used in 30 patients with spontaneous abortion the study group） and 30 individuals with artificial abortion （the control group）. The apoptosis index, the positive expression rates of Bax and Bcl-2 in villus syncytiotrophoblast cells were de- tected. All the data were analyzed by SPSS16.0. Results The apoptosis index of syncytiotrophoblast cells in the study group was （32.45±5.87）%, significantly higher than （19.38±4.16）% in the control group （P〈0.01）. The positive expression rate of Bax in the study group was significantly higher than in the control group, while that of Bcl-2 was significantly lower and Bel-2/Bax ratio was significantly higher （P〈0.01）. Condusion Intensive apoptosis, the in- creasing positive expression rate of Bax, and the decreasing positive expression rate of Bcl-2 in villus syncytiotropho- blast cells play critical roles in spontaneous abortion.