Sysmex XE-2100型血细胞分析仪异淋报警信息可信度评价

Evaluation on the credibility of the Sysmex XE-2100 hematology analyzer's abnormal lymphocytes alert

目的探讨Sysmex XE-2100型血细胞分析仪的异淋报警功能的可信度并确认人工阅片的意义。方法对284份外周血标本采用Sysmex XE-2100型血细胞分析仪进行血常规检查;并分别对Sysmex XE-2100型血细胞分析仪提示异淋阳性及异淋阴性的标本进行人工阅片(油镜分类200个白细胞,异淋比例>5%为阳性临界标准),记录并统计结果。结果 284份外周血标本Sysmex XE-2100型血细胞分析仪提示异淋阳性92份标本中,人工阅片异淋阳性23份,阳性预测率为25.00%,敏感度为60.53%;192份未提示异淋阳性的标本中人工阅片异淋阳性15份,阴性预测率为92.19%,特异度为71.95%。结论 System XE-2100型血细胞分析仪的预设异淋报警功能较差,导致人工阅片率偏高,厂家应根据全国各实验室实际情况重新调整或设置报警功能。

Objective To investigate the credibility of Sysmex XE-2100 hematology analyzer＇ s abnormal lymphoeytes alert, and to confirm the value of microscope. Methods We applied Sysmex XE-2100 hematology an- alyzer to carry out routine blood test on 284 venous blood samples. Blood smear microscope inspection was carried out to all the samples with lymphocytes alert. 200 leukocytes were counted in a randomly selected portion of the slide under oil light microscopy. Positive standard was determined as the ratio of abnormal lymphocytes more than 5%. All the data were collected and analyzed. Results Ninety-two positive samples of abnormal lymphocyte were reviewed by microscopy. The positive predictive rate was 25.00% （23/92）, and the sensitive rate was 60.53% （23/ 38）. 192 negative samples of abnormal lymphocyte were also reviewed by microscopy, of which 15 were found positive by manual re-examination. The negative predictive rate was 92.19% （177/192）, and the sensitive rate was 71.95% （177/246）. Conclusion Sysmex XE-2100 hematology analyzer does not have a satisfactory performance on lymphocytes abnormal alerting system, resulting in high manual re-examination rate. The fimction should be revised ac- cording to actual situation.