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拟南芥AtNUDT10启动子的分离及其功能分析 被引量：1

Cloning and Function Analysis of Promoter of AtNUDT10 in Arabidopsis thaliana
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摘要 根据已发表的拟南芥基因组序列设计合成一对引物,以拟南芥基因组DNA为模板克隆AtNUDT10上游非编码区,并与pBAR-GUS3相连构建了植物表达载体pNUDT10P-GUS,采用农杆菌GV3101介导的渗透法转化野生型拟南芥,通过除草剂筛选获得了一批抗性纯合子转基因植株。然后对转基因植株2周幼苗进行GUS活性的组织化学染色分析,并对3.5～4周的转基因植株叶片进行病原菌诱导表达分析。结果表明,已获得AtNUDT10启动子,该启动子为组成型表达的启动子,并且病原菌Pst.DC3000及Pst.DC3000AvrB对该启动子没有诱导作用。 5＇-UTR of AtNUDTIO was cloned by PCR from Arabidopsis thaliana and was inserted into pBAR-GUS to form a recombined construct pNUDTIOP-GUS. The construct was then used to transform wide-type Arabidopsis thaliana by Agrobacterium tumefaciens mediating. A batch of transgenic Arabidopsis thaliana with 5＇-UTR of AtNUDT10 was obtained through glufosinate screening. The results showed that, we had got the promoter of AtNUDT2 that was constitutive promoter. Furthermore, the expression of the promoter could not be induced by the Pst.DC3000 and Pst.DC3000 AvrB.
作者 张秀春 李若霖 唐文 夏亦荠 彭明 吴坤鑫
机构地区 中国热带农业科学院热带生物技术研究所/农业部热带作物生物学与遗传资源利用重点实验室 农业部转基因植物及植物用微生物环境安全监督检验测试中心 海南大学农学院
出处 《中国农学通报》 CSCD 2012年第21期164-168,共5页 Chinese Agricultural Science Bulletin
基金 国家自然科学基金(30760196) 中央级公益性科研院所基本科研业务费专项资金(中国热带农业科学院院本级)资助项目(1630052012007)
关键词 启动子 AtNUDT10 克隆 GUS染色 promoter AtNUDT10, clone GUS staining
分类号 S685.12 [农业科学—观赏园艺]
  • 相关文献

参考文献18

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  • 2Bessman M J, Frick D N, O'Handley S F. The MutT proteins or "Nudix" hydrolases, a family of versatile, widely distributed, "housecleaning" enzymes[J]. J Biol Chem, 1996,271(41): 25059-25062.
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二级参考文献12

  • 1MCLENNAN A G. The Nudix hydrolase superfamily[ J]. Cell Mol Life Sci. 2006,63 (2) :123 -143.
  • 2BESSMAN M J, Frick D N, O' HANDLEY S F. The MutT proteins or "Nudix" hydrolases, a family of versatile, widely dis- tributed, "housecleaning" enzymes [ J ]. J Biol Chem. 1996,271 (41 ) :25059 - 25062.
  • 3MCLENNAN A G, CARTWRIGHT J L, GASMI L. The human NUDT family of nucleotide hydrolases. Enzymes of diverse substrate specificity[ J]. Adv Exp Med Biol. 2000,486 : 115 - 118.
  • 4OGAWA T, UEDA Y, YOSHIMURA K, et al. Comprehensive analysis of eytosolic Nudix hydrolases in Arabidopsis thaliana [ J ]. J Biol Chem. 2005,280 (26) :25277 - 25283.
  • 5KLAUS S M, WEGKAMP A, SYBESMA W, et al. A nudix enzyme removes pyrophosphate from dihydroneopterin triphosphate in the folate synthesis pathway of bacteria and plants[J].J Biol Chem. 2005,280(7) :5274 -5280.
  • 6YOSHIMURA K, OGAWA T, UEDA Y, et al. At-NUDX1, an 8-oxo-7,8-dihydro-2'-deoxyguanosine 5'-triphosphate pyro- phosphohydrolase, is responsible foreliminating oxidized nucleotides in Arabidopsis[ J]. Plant Cell Physiol. 2007,48:1438 - 1449.
  • 7OLEJNIK K, KRASZEWSKA E. Cloning and charactarization of an Arabidopsis thaliana Nudix hydrolase homologous to the mammalian GFG protein [ J ]. Biochim Biophys Acta. 2005,1752 (2) :133 -141.
  • 8JAMBUNATHAN N, MAHALINGAM R. Analysis of Arabidopsis Growth Factor Gene 1 ( GFG1 ) encoding a nudix hydrolase during oxidative signaling[ J]. Planta. 2005 ( 3 ) : 1 - 11.
  • 9BARTSCH M, GOBBATO E, BEDNAREK P, et al. Salicylic Acid-Independent ENHANCED DISEASE SUSCEPTIBILITY1 Signaling in Arabidopsis Immunity and Cell Death Is Regulated by the Monooxygenase FMO1 and the Nudix Hydrolase NUDT7 [ J]. Plant Cell. 2006,18 : 1038 - 1051.
  • 10OGAWA T, ISHIKAWA K, HARADA K, et al. Overexpression of an ADP-ribose pyrophosphatase, AtNUDX2, confers en- hanced tolerance to oxidative stress in Arabidopsis plants[J]. The Plant Journal. 2009,57:289- 301.

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中国农学通报
2012年 第21期

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