应用正交设计优选秸秆降解菌组合

Optimization of Combination of Straw-degrading Bacteria by Orthogonal Design

为了探索利用正交设计筛选秸秆降解菌组合的方法,优选产纤维素酶和木质素酶的秸秆降解菌的组合。利用正交设计以玉米秸秆粉为发酵底物,检测分析不同组合的降解率及酶活;采用平板拮抗试验测定组合内菌株间的拮抗作用,检测筛选的效果。结果表明,降解菌不同组合之间玉米秸秆降解率存在着极显著的差异。筛选出最佳组合为A1B3C1D4E1F2G1,即接种量F-11为1mL,XX-10为2.0mL,G-09为1.0mL,降解率超过对照10%以上。部分降解菌菌株之间存在一定的拮抗作用,秸秆降解率与组合内降解菌株的组成有关。正交试验设计是筛选秸秆降解菌最佳组合的一种有效方法,筛选出的降解菌最佳组合秸秆降解能力强,具有较好的开发利用价值。

To explore the application of orthogonal design in screening combination of straw-degrading bacteria, optimize combination of straw-degrading bacteria producing cellulase and ligninase. With corn straw as a fermentation substrate, the straw-degrading rate and enzyme activity of different combinations of degrading bacteria were investigated with orthogonal design. The antagonism between strains in selected combinations was studied with plate test. Results showed that, the difference of straw-degrading rates among different treatments was extremely significant. The optimal combination was A1B3C1D4E1F2G1, i.e., the inoculation volume of stain F-11, XX-10 and G-09 was 1 mL, 2.0 mL and 1.0 mL, respectively. The degradation rate increased by 10% compared with that straw-degrading bacteria, and the straw-degrading rate orthogonal design was an effective method to screen the of the control. The antagonism existed between some was related to strain composition in combination. The best combination of straw-degrading bacteria, and the selected bacteria combination had high degrading efficiency for straw and very good value of development and utilization.