克氏伪裸头绦虫成虫染色标本的制作及其形态学观察

Preparation of Stained Specimens for the Adult of Pseudanoplocephala crawfordi and Its Structural Observation

为了弄清克氏伪裸头绦虫的形态结构特征。用苏木素染色和齐尔石碳酸复红染色制作了猪源克氏伪裸头绦虫成虫染色标本,并对其进行形态学观察。结果显示:颈节、幼节和成节用15倍苏木素稀释染液染色12h效果最佳,孕节以齐尔石碳酸复红染液染色24h为最佳。克氏伪裸头绦虫成虫呈扁平状乳白色,所有节片纵轴小于横轴,从幼节到成节开始逐渐出现睾丸、贮精囊、阴茎囊、卵巢、卵黄腺、子宫和生殖孔等生殖器官。孕节内充满虫卵,睾丸、卵巢、贮精囊等器官退化,生殖孔未退化。研究结果补充了寄生虫实验教学标本库,为猪和人克氏伪裸头绦虫病控制提供了重要参考依据。

To reveal the characteristic structures of Pseudanoplocephala crawfordi,the stained specimens of the adult of P.crawfordi from pigs were prepared with Hematoxylin staining and Ziehl phenol-basic fuchsin staining.The results showed that the optimal staining condition for collum segment,immature and mature proglottids was in the 1：15 diluted hematoxylin dye solution at room temperature（RT） for 12 hours,and that of gravid proglottids was in Ziehl phenol-basic fuchsin dye solution at RT for 24 hours.Adults of the parasite was flat and white,the vertical axises of proglottids were shorter than the lateral ones.Testicles and sperm pocket began to show in the immature proglottids,and while the complete male and female organs of generation including testicles,sperm pocket,penis pocket,ovary,yelk gland,uterus and gonopore occurred in the mature ones.Gravid proglottids were filled with eggs,while without other organs of generation with the exception of a gonopore.These findings provided a important reference for control of porcine and human pseudanoplocephalasis and supplement the specimen library of parasitological research and teaching.