溶剂梯度结合流速梯度洗脱—高效液相色谱法快速测定食用植物油中四种抗氧化剂

Fast determination of four antioxidants in edible vegetable oil by solvent gradient combined with velocity gradient elution-HPLC

该研究建立一种溶剂梯度结合流速梯度洗脱―高效液相色谱法快速测定叔丁基羟基茴香醚（BHA）、二丁基羟基对甲酚（BHT）、没食子酸丙酯（PG）、特丁基对苯二酚（TBHQ）四种抗氧化剂分析方法。试验以甲醇―0.5%乙酸溶液（v/v=65/35）为流动相进行梯度洗脱,并结合流速梯度洗脱,在10 min内分离上述四种物质。该法线性范围为0.25~250μg/mL,最小检测限为0.1~0.5μg/mL,线性相关系数为0.9995~0.9999,平均回收率为90.9%~96.1%,相对标准偏差（RSD）〈5.0%（n=5）。该法灵敏度高、重现性好、操作方便、检测快速,可作为一种检测食品抗氧化剂有效方法。

A method has been developed for fast determination of four antioxidants by solvent gradient combined with velocity gradient elution-high performance liquid chromatography, such as butyl hydroxy anisd （ BHA ）, 2,6-butylated hydroxytoluene （ BHT ）, tertiary butyl hydroquinone （ TBHQ ） and propyl gallate （ PG ）. Methanol and 0.5% ethylic acid （ v/v=65/35 ）was used as mobile phase using a solvent gradient elution combined with velocity gradient elution. Under the optimization conditions, the four antioxidants were separated within 10 min. The linear range was between 0.25 and 250 gg/mL, the detection limits were ranged from 0.1 to 0.5 gg/mL, the linear correlation coefficient was between 0.9995 and 0.9999, the average recovery was 90.9%~96.1%, and the relative standard deviation （ RSD ） was less than 5.0% （ n=5 ）. The method had advantages of high sensitivity, good reproducibility, simple operation, rapid determination, and it was an effective method for determining antioxidants in samples.