蟾酥注射液对新城疫疫苗免疫效果的影响研究

The Effects of Bufonis Parenteral on Immunity Effect of Newcastale Disease Vaccine

为了探讨蟾酥注射液对新城疫疫苗免疫效果的影响。选取1日龄健康雏鸡240只,随机分成4组,每组60只,设试验组(低剂量组、中剂量组和高剂量组)和对照组,选择不同浓度的蟾酥注射液与新城疫疫苗联合使用作为试验组。分别在21、28、35、42、49、56日龄,通过β-微量法,检测血清中ND-HI抗体;通过MTT法,检测外周血T细胞吸光度值(OD值);通过称量体重和免疫器官重量的方法,检测免疫器官指数。结果显示:蟾酥注射液各组与对照组比较,ND-HI抗体水平、外周血T细胞转化、胸腺指数、法氏囊指数和脾指数均有不同程度的提高。说明蟾酥注射液能够提高机体的体液免疫和细胞免疫水平,从而增强新城疫疫苗的免疫效果。

In order to study the effects of Bufonis parenteral on immunity effect of Newcastale disease vaccine, 240 one-day-old healthy chickens were randomly divided into control group and treatment group （low dosage groups, medium dosage groups and high dosage groups）, each group had 60 chickens. The different concentration combining usage of Bufonis parenteral solutions and Newcastale disease vaccine were selected as the treatment group. At the 21st, 28th, 35th, 42nd, 49th and 56th day, using/3-micromethod, tested ND-HI antibody in the serum; using MTT method, tested peripheral blood T-cell absorbance; using the method of weighing body weight and immune organ weight, tested immune organs index. The resuhs showed that the ND-HI antibody level, peripheral blood T-cell transformation, thymus index, bursal index and spleen index of Bufonis parenteral groups were improved to varying degrees comparing to control group. The resuks indicated that Bufonis parenteral could improved the body humoral immune and cellular immune level, thus enhance Newcastle disease vaccine immune effect.