亚抑菌浓度鱼腥草醇提物对金黄色葡萄球菌α-溶血素分泌的影响研究

The Influence of Subinhibitory Concentration of Ethanol Extracts from Houttuyniae cordata on α-toxin Secretion by Staphylococcus aureus

为了研究鱼腥草醇提物对金黄色葡萄球菌的抗菌活性以及其对金葡菌α-溶血素分泌的影响。通过最低抑菌浓度的测定并应用溶血试验、Western-blot分析、荧光定量PCR试验以及细胞毒性试验考察亚抑菌浓度的鱼腥草醇提物对金葡菌α-溶血素分泌的影响。结果表明：鱼腥草醇提物对9株金葡菌的最小抑菌浓度范围为16~64mg/mL,亚抑菌浓度鱼腥草醇提物呈剂量依赖式抑制金葡菌α-溶血素的分泌,尤其当浓度为0.16mg/mL时,基本上能完全抑制金葡菌α-溶血素的分泌。本研究可能为抗金葡菌感染提供一种新的策略。

In this paper, we have determined the minimal inhibitory concentrations （MICs） of ethanol extracts from Houttuyniae eordata for staphylococcus aureus and hemolysis assay, Western-blot, Real-time RT-PCR and cytotoxieity assay were further employed to elucidate the antimicrobial activity of this extract against S. aureus and the influence of subinhibitory concentration of this extract on a-toxin production. The MICs of ethanol extracts from Houttuyniae cordata for 9 S. aureus strains were ranged from 16 to 64 mg/mL. Subinhibitory concentration of ethanol extracts from Houttuynlae cordata reduced the production of a-toxin by S. aureus in a dose-dependent manner. In particular, 0.16 mg/mL of ethanol extracts from Houttuyniae eordata completely suppresses the production of a-toxin. This study might provided a new strategy for anti-Staphylococcus aureus infection.