荚果蕨中多糖的超声辅助提取及其DPPH自由基清除作用的研究

Ultrasonic extraction of polysaccharides from Matteuccia struthiopteris and their DPPH free radical scavenging activity

以荚果蕨(Matteuccia struthiopteris(L.)Todaro)根状茎为材料,采用单因素实验和正交实验探讨超声辅助提取多糖的最佳工艺条件,应用1,1-二苯基苦基苯肼(DPPH)自由基清除法评价荚果蕨根状茎中多糖的抗氧化活性。实验结果表明,超声辅助提取该多糖的最佳工艺参数为:提取温度为80℃、料水比为1:50(g/mL)、超声时间为30min、超声功率为150W。在此条件下提取的荚果蕨根状茎、营养叶、孢子叶3个部位中粗多糖的提取率分别为9.67%、3.30%、3.43%。同时,荚果蕨根状茎中多糖有较强的抗氧化作用,对DPPH自由基半数抑制浓度(IC50)为68.68μg/mL。研究荚果蕨多糖的超声辅助提取工艺及抗氧化活性,对荚果蕨天然保健品的开发利用有重大指导意义。

The condition for ultrasonic extraction of polysaccharides from Matteuccia struthiopteris were optimized by one-factor-at-a-time and orthogonal array design methods.Polysaccharide from Matteuccia struthiopteris was tested with DPPH· to estimate their antioxidative activity.The results showed that the best extraction conditions were determined as below：ethanol liquid ratio was 1：50（g/mL）,extraction temperature was 80℃,ultrasonic power was 150W,ultrasonic time was 30min.The extraction yield of polysaccharide in rhizome,trophophyll and sporophyl was 9.67%,3.30% and 3.43% respectively.The polysaccharide had high antioxidative activity,revealing an IC50 of 68.68μg/mL.Investigating ultrasonic traction of polysaccharides from matteuccia struthiopteris and their DPPH free radical scavenging activity,it is a great direction significance in the development and utilization of matteuccia struthiopteris.