摘要
采用聚乙二醇/硫酸铵[PEG/(NH4)2SO4]建立稳定的双水相体系以分离猪胃蛋白酶。通过上下相体积比(VR)、比活力(SA)、纯化因子(PF)、酶活回收率(η)和SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)分析测定萃取效果。结果表明,萃取猪胃蛋白酶的最佳配比体系为25%PEG1000:18%(NH4)2SO4,所萃取的猪胃蛋白酶酶活回收率为91.5%,SDS-PAGE结果显示,萃取的蛋白质和猪胃蛋白酶对照品分子量大小一致。
The pepsin was extracted and purified by PEG/（NH4）2SO4 aqueous two-phase system（ATPS）from stomach of swine.Extraction technology of pepsin using a newly established ATPS was investigated according to volume ratio（VR）,purification factor（PF）,specific activity（SA）,protease activity recovery yield（η）and SDS-polyacrylamide gel electrophoresis（SDS-PAGE）.The optimal ATPS conditions for pepsin partitioning was 25% PEG1000：18%（NH4）2SO4,which activity recovery yield reached 91.5%.SDS-PAGE indicated that ATPS fraction had a major protein which molecular weight（MW）was consistent with swine pepsin.
出处
《食品工业科技》
CAS
CSCD
北大核心
2012年第15期245-247,共3页
Science and Technology of Food Industry
基金
湖南省教育厅项目(11C0329)
