离子交换法提取酶促转化液中的L-茶氨酸

Purification of L-theanine from enzymatic catalyzed reaction solution by ion-exchange resins

用717阴离子交换树脂提取酶促转化液中的L-茶氨酸,用静态和动态的方法考察了不同操作条件对固定床离子交换效果的影响。静态吸附实验表明,717阴离子交换树脂对酶促转化液中L-茶氨酸的最佳吸附条件为pH8.5,平衡吸附时间16min,吸附量为0.083mmol/g。L-茶氨酸转化液上样的最佳流速为0.5BV/h。按照以上条件,转化液稀释3倍上样,经0.5mol/L盐酸洗脱,旋转蒸发仪浓缩,醇沉结晶后,可得L-茶氨酸纯品。

L-theanine was isolation and purification from enzymatic catalyzed reaction solution by 717 anion-exchange resins.Through static and dynamic experiments,different operating conditions in the fixed bed to separate L-theanine were investigated.The results showed that the optimal conditions were pH8.5,adsorption balance time was 16min and exchange capacity was 0.083mmol/g in static experiment.The optimal flow rate was 0.5BV/h in dynamic experiment.Under these conditions,the reaction solution was diluted with water,added into the ion exchange column and eluted by 0.5mol/L hydrochloric acids.White crystal of L-theanine was obtained after concentrated,crystallized and dryied.