响应曲面法优化蓝莓渣多酚提取工艺

Process optimization for polyphenol extraction from blueberry marc by response surface methodology

实验采用乙醇提取蓝莓渣多酚。在单因素实验的基础上,使用DesignExpert7.0软件设计,通过Plackett-Burman实验及响应曲面实验分析确定了乙醇提取蓝莓渣多酚物质的最佳工艺条件为:乙醇浓度60%,液料比20(mL/g),提取温度80℃,pH2,蓝莓渣多酚提取率为97.01%。

Polyphenols were extracted by ethanol solvent from blueberry marc.The results of single factor experiment on extraction conditions were designed by Design-Expert 7.0 software and optimized by experiments of Plackett-Burman and response surface methodology.The best extraction conditions were confirmed as follow：ethanol concentration was 60%,solvent/material ratio was 20（mL/g）,extraction temperature was 80℃,pH was 2.Under these conditions,the percent of extracted polyphenols was 97.01%.