徽州贡菊总黄酮对D半乳糖致衰老小鼠的抗氧化作用

Antioxidant effect of total flavonoids from Huizhou Gongju on aging mouse induced by D-galactose

目的:探讨徽州贡菊总黄酮对衰老小鼠抗氧化作用的影响。方法:D-半乳糖致衰老模型法,采用200、400、800mg/(kg·dbw)剂量的徽州贡菊总黄酮给小鼠灌胃,检测血清和肝中超氧化物歧化酶(SOD)活性、谷胱甘肽过氧化物酶(GSH-Px)活性、过氧化氢酶(CAT)活性、总抗氧化能力(T-AOC)和血清、肝及脑中的MDA含量。结果:与模型组比较,不同剂量组能显著提高血清和肝组织中SOD、GSH-Px、CAT、T-AOC活性,降低血清、肝脏和脑组织中MDA含量。结论:徽州贡菊总黄酮具有一定的抗氧化功能。

Objective：To research the anti-oxidative activity of total flavonoids from Gongju（GTF）in aged mice.Method：GTF was administrated by gavage[200,400,800mg/（kg·d bw）]to mice for 30d.The mice were then sacrificed,and the serum,liver and brain tissue were collected.The concentration of malondialdehyde（MDA）in the blood,liver and brain tissue and the activities of superoxide dismutase（SOD）,glutathione peroxidase（GSH-Px）,Catalase（CAT）,total antioxidant activity（T-AOC）in the serum and liver were then measured.Result：GTF produced a dose-dependent decrease of MDA in the serum,liver and brain tissue,at the same time a dose-dependent increase of activities of SOD,GSH-Px,CAT and T-AOC at all three doses in the blood and liver.Conclusion：GTF had antioxidant activity by protecting body against free radical-induced damage.