不同方法检测尿红细胞的对比分析

Investigation of the attention points of the urinary erythrocytes test by using different methods

目的探讨不同方法检测尿红细胞时的差异性,比较不同仪器检测方法对红细胞尿的携带污染率情况。方法对来自临床1 000例尿液标本分别用URISYS2400干化学分析仪、UF-500i尿有形成分分析仪、显微镜镜检进行检测。对健康成人尿标本和与之混合的不同浓度红细胞悬液交叉排列分别用干化学法和尿沉渣法进行检测。结果分别用干化学法、尿沉渣法、显微镜镜检检测尿红细胞的阳性率为41.1%(411/1 000)、22.6%(226/1 000)、19.7%(197/1 000),干化学法和尿沉渣法分别与显微镜镜检比较复合率分别是76.8%(768/1 000)、97.1%(971/1 000)。干化学和尿沉渣同时红细胞检测阴性为512例,采用显微镜复检均为阴性。携带污染率URI-SYS2400干化学分析仪为0.000%、UF-500i尿有形成分析仪为0.113%。结论干化学法、尿沉渣法检查尿红细胞可以提高检测效率,两者相互补充可以提高检测的准确度,但仍有影响因素不可避免,所以当两者检测不符时,仍然需要显微镜镜检确认。

Objective To explore the difference of the different instruments in the urinary erythrocytes test, and to compare situations of contamination rates of urinary erythrocytes test by different test methods. Methods 1 000 urine samples were tested by the URISYS2400 dipstick analyzer, the UF-500i urine sedimentary analyzer and microscope. Healthy adult urine specimens and these mixed randomly the erythrocytes suspensions with different concentrations were tested by the dipsticks analyzer and urine sediment analyzer. Results The positive rates of dip- stick analyzer, urine sediment analyzer and the microscopic examination were 41.1 % （411/1 000）, 22.6 % （226/1 000） and 19.7 % （197/1 000）, respectively. The matching rates of dipsticks analyzer and urine sediment analyzer were 76. 8%（768/1 000） and 97.1%（971/1 000） comparing to microscopic examination..512 samples detected as negative a- bout the urine erythrocytes test by both the dipstick analyzer and the urine sedimentary analyzer were also negative by microscopy examination. The experiment showed that the carryover rates were 0. 000% for URISYS2400 dipsticks analyzer and 0. 113% for UF-500i urine sedimentary analyzer. Conclusion The detective effectiveness of the urine erythrocyts test can be greatly improved by using the dipstick and the urine sedimentary analyzer. The accuracy of the test can be increased by the complementary test using both two instruments. The confirmation of microscopy is still unavoidable when their results can not match.