镇脑宁胶囊对动物脑微循环的影响

Effects of Zhennao-Ning capsule on cerebral microcirculation

目的观察镇脑宁胶囊对动物脑微循环的影响。方法采用结扎右侧颈总动脉法建立小鼠脑缺血模型，描记造模前5min、造模后10、20、30min小鼠脑血流量，计算血流量下降率；采用静脉注射高分子右旋糖苷法致大鼠脑微循环障碍，记录出现微循环障碍的时间，以及注射20min后同一视野的血管流速、血液流态以及网点数。结果结扎右侧颈总动脉后，脑缺血小鼠于10、20、30min大脑皮层血流量明显下降，分别为（71±13）％、（71±15）％和（74±16）％（P〈0．01）；与模型组比较，镇脑宁胶囊1500mg以（g和750mg／kg给药后，在结扎后10、20、30min内，小鼠大脑皮层血流量均有明显增加（56％、51％、50％和42％、49％、51％，P〈0．01）。注射高分子右旋糖苷后，微循环障碍模型组大鼠的血液流速差为（572±177）gm／s，出现微循环障碍时间为（232±70）s；镇脑宁胶囊600mg／kg和300mg／kg给药后均能使血液流态改变减轻（P〈0．01），血液流速分别增加26％，36％，出现微循环障碍的时间分别延长53％，34％（P〈0．01或P〈0．05）。结论镇脑宁胶囊通过改善动物脑微循环治疗偏头痛。

Objective To observe the effects of Zhennao-Ning capsule on cerebral microcirculation. Methods Cerebral hypoperfusion model of mices was induced by fight common carotid arteries ligation method, The cerebral blood flow for 5rain was recorded under normal circumstances comand for 30rain when modeled, and then calculate the rate of blood flow changes. Microcirculatory impairment model of rats was induced by intravenous injection of high molecular dextran（HMD）. The time of the emergence of microcirculatory impairment was recorded. Vascular flow, blood flow pattern and the number of outlets in the same range were recorded. Results The blood flow was significant decrease in the cerebral hypoperfusion model mice, when compared with the control mice. Zhennao-Ning capsules at the dosage of 1500 mg/kg, 750 mg/kg treatment could improve the cerebral blood flow of rats at 10, 20, and 30 min after modeled （56%, 51%, 50% and 42%, 49%, 51%, respectively, P〈0.01）. Zhermao-Ning capsules at the dosage of 600 mg/kg, 300 mg/kg could significantly reduce the change of blood flow pattern（P〈0.01）, improve vascular flow rate （26%, 36%, P〈0.05 or P〈0.01）, extend the time of the emergence in the microcirculatory impairment model rat （53%, 34%, P〈0.01）. Conclusion Zhennao-Ning capsules could improve microcirculatory impairment and partly cure the clinical migraine disease.