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过氧亚硝酸盐对糖尿病大鼠血管收缩功能的影响的研究 被引量：6

Influence of peroxynitrite on vasoconstrictive function in diabetic rats
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摘要 目的观察过氧亚硝酸盐对糖尿病大鼠胸主动脉血管收缩功能的影响。方法雄性SD大鼠30只,大鼠随机分为3组:对照组、糖尿病组和糖尿病+尿酸盐孵育(过氧亚硝酸盐清除剂)组。以去除内皮的大鼠胸主动脉作为实验标本。分别检测各组血管收缩功能、RhoA/ROCK表达水平和硝基酪氨酸表达水平的差异。结果糖尿病组血管的收缩反应增强。糖尿病组与尿酸盐孵育组相比,RhoA mRNA表达水平升高。糖尿病组血管ROCK的蛋白表达水平显著升高。糖尿病组血管硝基酪氨酸表达水平显著升高。结论过氧亚硝酸盐导致糖尿病大鼠胸主动脉血管ROCK蛋白表达水平增加及血管收缩反应增强。 Objective To observe the influence of peroxynitrite （ONOO-） on vasoconstrictive function of tho- racic aorta in diabetic rats. Methods Male SD rats （n = 30） were randomly divided into control group, diabetes group and diabetes ＋ urate incubation （ peroxynitrite scavenger） group （ urate group）. Taking rat thoracic aorta after re- moving endothelium cells as test sample, the differences in vasoconstrictive function, and expressions of RhoA/ROCK and nitrotyrosine were detected in all groups. Results The vasoconstrictive response was increased in diabetes group. The expression of RhoA mRNA was higher in diabetes group than that in urate group. The expressions of ROCK protein and nitrotyrosine increased significantly in diabetes group. Conclusion Peroxynitrite can increase the ex- pression of ROCK protein and promote vasoconstrictive response in rats with diabetes induced by peroxynitrite.
作者 王宇朋 王萍 李敏 陈海平 王翠英 李虹伟
机构地区 首都医科大学附属北京友谊医院医疗保健中心 首都医科大学附属北京友谊医院心脏中心
出处 《中国循证心血管医学杂志》 2012年第3期254-257,共4页 Chinese Journal of Evidence-Based Cardiovascular Medicine
关键词 过氧亚硝酸盐 糖尿病 大鼠 Peroxynitrite Diabetes Rats
分类号 R544.1 [医药卫生—心血管疾病]
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参考文献9

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同被引文献55

  • 1于德民,吴锐,尹潍,袁咏.实验性链脲佐菌素糖尿病动物模型的研究[J].中国糖尿病杂志,1995,3(2):105-109. 被引量：425
  • 2黄敬泽,王健.2型糖尿病糖化血红蛋白与全天不同时间血糖水平的关系[J].中国综合临床,2006,22(12):1101-1103. 被引量：42
  • 3Thakali K, Demel SL, Fink GD, et al. Endothelin - 1 - induced cont- ration in veins is independent of hydyogen peroxide[J]. Am J PhysiolHeart Circ Physiol, 2005,289 (3) : H1115 - H1122.
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  • 7Wang Y, Zheng XR, Riddick N, et al. ROCK isoform regulation of my- osin phosphatase and contractility in vascular smooth muscle cells [ J ]. Circ Res, 2009,104 (4) : 531 - 540.
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  • 9Himki J, Shimokawa H, Higashi M, et al. Inflammatory stimuli upreg- ulated Rho - kinase in human coronary vascular smooth muscle cells [J]. J Mol Cell Cardiol,2004,37(2):537 -546.
  • 10American Diabetes Association. Diagnosis and classification of diabetes mellitus [J]. Diabetes Care, 2009, 32 (Suppl 1):S62.

引证文献6

二级引证文献11

中国循证心血管医学杂志
2012年 第3期

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