阿萨希毛孢子菌胞外分泌酶水平的比较

Comparison of the secretory level of extracellular protease from Trichosporon asahiis

目的探索阿萨希毛孢子菌细胞外分泌酶的种类与活性。方法采用卵黄琼脂培养基、BSA-琼脂培养基和AIP-ZYM试剂盒,观察11株阿萨希毛孢子菌细胞外分泌酶的种类及酶活性。结果 11株阿萨希毛孢子菌中9株磷脂酶活性较强,2株酶活性弱;均未测得酸性蛋白酶分泌。AIP-ZYM试剂盒显示11株菌有共性分泌酶,也有部分菌株有特异性分泌酶。结论阿萨希毛孢子菌细胞外分泌酶种类多样,不同来源菌株产酶种类及酶活性亦不相同。

Objective To explore the type and the activity of extracellular protease from Trichosporon asahiis（T.asahii）. Methods A series of assays including Egg Yolk Agar, BSA-agar meddium and AIP-ZYM kit were used to observe the extracellular proteases from Zasahii. Result Nine of the total 11 strains presented higher phospholipase activity than other 2 strains. Acid protease was not detected in all the strains. All the 11 strians possesed certain extracellular proteases of the same species, meanwhile some strains had specific extracellular protease. Conclusion Extracellular protease from T.asahii are multiplex, for the different strains, the type of extracellular protease and the activity of enzymatic activity is different.