亚甲基四氢叶酸还原酶基因和血压水平的遗传流行病学研究-同胞对连锁分析

An genetic epidemiology study of MTHFR gene and blood level (sibpair linkage analysis)

目的 探索 5 ,10 亚甲基四氢叶酸还原酶 (MTHFR)基因与血压水平的连锁关系。方法 研究现场选择在原发性高血压社区综合防治项目农村社区 -北京市房山区良各庄村和黄山店村。研究对象为该现场的 142对同胞对 ,及可能获得的父母。基因型测量包括血凝块中提取DNA ,PCR扩增目的基因片断 ,酶切分析MTHFR基因多态性。表型为血压水平 ,协变量的测量包括问卷调查、体格指标及血液生化指标的测量。统计分析方法为数量型同胞对连锁分析 ,分析软件为SAGE。结果 在研究人群MTHFR基因 6 77位C和T等位基因频率分别为 0 .70和 0 .30 ,基因型频率 (CC、CT、TT)分别为 49.7%、41.0 %和 9.3%。没有发现MTHFR基因与血压水平存在连锁 (P >0 .0 5 )。结论 本研究人群MTHFR基因不太可能是决定血压水平的主基因。

Objective To explore the relationship between MTHFR gene and blood pressure. Methods A total of 142 sibpairs were collected from rural communities of CCPACH. MTHFR gene genotypes were determined by an assay based on the polymerase chain reaction. Phenotype of this study was the level of blood pressure. Covariates were measured by questionnare, physical examination and clinical indices. Sibpair linkage analysis was used to analyse data by SAGE software. Results In this study, prevalence of TT genotype of MTHFR gene was 9.3 %, while CT was 41%. There was no evidence for linkage of the MTHFR gene with either systolic or diastolic blood pressure identified. Conclusion In our study population, MTHFR gene did not seem to be not related to blood pressure.