斑点热群立克次体HN-98株的分离和初步鉴定

Isolation and identification of HN-98 strain of spotted fever group rickettsiae

目的 从海南省斑点热疫源地不明热病人体内分离斑点热群立克次体。方法 用鸡胚卵黄囊培养法直接从斑点热疫区一不明热病人血标本中分离立克次体 ,继之用分子生物学及血清学试验对分离株进行鉴定。结果 从海南省琼中县斑点热疫区一不明热男性民工的血标本中分离到了一株立克次体 ,命名为HN 98株立克次体 ,西伯利亚种。结论 该结果进一步从病原学上证实海南省琼中县存在有斑点热疫源地。

Objective One strain of rickettsiae was isolated from a patient with unknown fever from Qiongzhong county in Hainan Provinve in 1998, using embryonated hen eggs and proved to be the member of rickettsiae by the methods of morphology. It was named HS 98 strain after the name of the place and the year it was isolated.Methods The isolate was identified by methods of Microcomplement fixation assay and OCR/RFLP and compared with known species and strains of SFGR by DNA polymerase chain reaction and DNA polymerase chainreaction and was followed by restriction endonuckease fragment length polymorphism analysis. Results Results demonstrated that HN 98 strain was antigenically and genotypically identical to R.sibirica 246. Conclusion The results suggested that there existed natural foci of spotted fever in Qiongzhong Area of Hainan Province.