慢性间断性低氧对发育期幼鼠认知功能的影响及机制研究

Influence of chronic intermittent hypoxia on cognitive function in puberty rats and mechanism research

目的探讨慢性间断性低氧对发育期幼鼠认知功能的影响及其可能机制。方法 21日龄SD雄性幼鼠20只,随机均分为对照组、慢性间断性低氧(CIH)组。CIH组幼鼠在自制缺氧舱内每天缺氧8 h,连续缺氧6周。利用Mor-ris水迷宫检测幼鼠认知功能的变化;利用HE染色在光镜下观察海马神经元组织学结构变化。结果与对照组比较,CIH组幼鼠出现认知功能障碍(P<0.05);对照组海马神经元形态正常,CIH组海马神经元出现变性坏死。结论 CIH致发育期幼鼠认知功能障碍,其机制可能与海马神经元病理损伤有关。

Objective To investigate the influence of chronic intermittent hypoxia on cognitive function in puberty rats and the relative mechanism. Methods 20 postnatal 21 d （P21） Sprague-Dawley （SD） male rats were divided into two groups ran- domly and averagely, the control group and the chronic intermittent hypoxia（CIH） group. The rats in the CIH group were exposed to intermittent hypoxia in self-designed cabin for 8 h every day, and the duration of hypoxia was 6 weeks. The cognitive function was assessed by the Morris Water Maze. The morphologic changes in hippocampus were observed. Results Compared with the contro! group, the cognitive impairment in the CIH group was statistically significant （P〈0.05）. Degeneration or necrosis neurons in hippocampus were observed in CIH rats. Conclusion The study demonstrates that CIH exposures result in significant cogni- tive impairment of puberty rats, which may be associated with the necrosis of neurons in hippocampus.