脑源性神经营养因子在猫备用背根节的表达变化

Expressional Change of Brain-Derived Neurotrophic Factor in Adult Cat Spared DRG

为探讨脑源性神经营养因子（BDN）在备用背根节（DRG）的表达变化情况，对20只成年雄性家猫（其中15只行单侧L6备用根手术后分别存活3、6、12天）L6DRG行BDNF免疫组化染色。结果：①正常猫L6DRG神经元以胞体直径32μm和44μm为分界划分出大中小三类；②正常L6DRG内有50．87％的神经元呈BDNF阳性反应，备用根手术侧其BDNF阳性神经无数量增多，反应增强，以术后6天变化最显著（76．7％）。正常及术后BDNF阳性神经元均以小神经元为主。去部分背根后备用L6DRG神经元BDNF表达的增多、增强可能参与促进其神经元（尤其是小神经元）中枢支在脊髓背角的侧支出芽，并可能参与营救一些失去部分神经支配的背角神经元。

This investigation was aimed to search the expressional change of BDNF in the normal and spared L6DRG. The L6DRGs from 20 adult male cats (5 normal cats, 15 unilateral L6spared roots cats allowed to survive 3 days, 6 days and 12 days respectively) were embedded in paraffin, using immunohistochemical reaction to detect BDNF. Results: 1. Normal DRG neurons were divided into three categories (large, medium and small )with the cell body diameter 32μm and 44μm as the dividing lines. 2. There are 50. 87% BDNF positive neurons in normal L6 DRG. After operation, the number of BDNF positive neurons of the operated side DRG significantly increased,especially 6 days (76. 70% ). Normal and postoperative BDNF positive neurons were mainly small neurons. After partial section of DRG, the expression of BDNF in spared DRG neurons increased, which might be involved in promoting collateral sprouting of spared root in spinal cord and rescuing the dorsal horn neurons from partial deafferentation.