成都地区汉族人群ACE基因多态性与血清ACE活性的相关性

Polymorphism of ACE Gene and Its Relationship with Serum ACE Activity in the Hans in Chengdu Region

为调查中国汉族人群血管紧张素转换酶（ACE）基因多态性分布及与血清ACE（SACE）活性的相关性。采用比色法和PCR技术，对90例健康汉族人群进行SACE活性和ACE基因插入／缺失（I／D）多态性的检测。结果显示：中国汉族人群ACE基因DD型占18％，DI型占44％,Ⅱ型占38％，D和Ⅰ等位基因频率分别为0．40和060。ACE基因多态性与SACE活性密切相关，提示不同种族间ACE基因分布存在着差异，中国汉族人群ACE基因多态性以Ⅱ型和Ⅰ等位基因占优势；SACE活性受ACE基因Ⅰ／D多态性的影响．

This study was aimed at the angiotensin converting enzyme (ACE) gene insertion/deletion (I/D) polymorphism distribution in Chinese and its relationship with serum ACE activity(SACE). SACE activity and ACE gene polymorphism in 90 healthy persons in Chengdu region were investigated using a spectrophotometric assay and the PCR technique, respectively. The results showed that, in the Hans, DD genotype of ACE gene was 18%, DI genotype 44%, and Ⅱ genotype 38%. Allele frequencies were 0. 40 for the D allele and 0.60 for the Ⅰ allele. There were significant relationships between ACE I/D polymorphism and SACE activity. The results suggest that ACE genotype distribution be different between races, that Ⅱ genotype and Ⅰ allele be more frequent in Chinese, and that SACE activity be under the influence of a genetic polymorphism of ACE in Chinese.