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辛伐他汀预处理对脓毒症大鼠血清血管性血友病因子的影响 被引量：2

Effect of simvastatin pretreatment on serum von Willebrand factor in a rat model of sepsis
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摘要 目的评价辛伐他汀预处理对脓毒症大鼠血管内皮细胞功能的影响。方法清洁级雌性Wistar大鼠96只,体质量200～250g,4月龄。采用随机数字表法,将大鼠随机分为3组:假手术组32只、脓毒症组32只和辛伐他汀组32只。脓毒症组和辛伐他汀组采用盲肠结扎穿孔法制备脓毒症模型。辛伐他汀组制模前胃内灌注辛伐他汀20mg.kg-1.d-1,每日1次,连续2周;假手术组和脓毒症组胃内灌注等容量生理盐水。分别于术后3、6、24和48小时取8只大鼠,采集颈动脉血样,测定白细胞计数;采用酶联免疫吸附测定(ELISA)法检测血清血管性血友病因子(vWF)水平;记录术后3小时内、>3～6小时、>6～24小时和>24～48小时各时段大鼠的死亡情况。结果与假手术组比较,脓毒症组和辛伐他汀组术后各时点白细胞计数和血清vWF浓度升高,术后6小时达峰值,白细胞计数分别为(7.45±0.69)×109/L,(12.79±0.51)×109/L,(10.36±0.19)×109/L;vWF水平分别为(0.543±0.001)μg/L,(1.198±0.035)μg/L,(1.018±0.005)μg/L(P<0.05);与脓毒症组比较,辛伐他汀组术后各时点白细胞计数和血清vWF水平降低,术后24～48小时内病死率降低(脓毒症组75.0%,辛伐他汀组50.0%)(P<0.05)。结论辛伐他汀可保护脓毒症大鼠的内皮细胞功能,从而降低48小时内的病死率。 Objective To evaluate the effect of simvastatin pretreatment on endothelial cell function in a rat model of sepsis. Methods Ninety six pathogen-free female Wistar rats aged 4 months weighing 200 250 g were randomly divided into three groups：Sham operation group （ n = 32）, sepsis group （ n = 32） and simvastatin group （n= 32）. Sepsis was induced by cecal ligation and puncture（CLP）. In simvastatin group, simvastatin 20 mg/kg was given via gastric tube once a day for two weeks before CLP. Sham operation group and sepsis group were given isasteric saline. Blood samples were taken from carotid artery at 3 h,6 h,24 h and 48 h after operation（ n =8 at each time point） for WBC count and measurement of serum vWF concentration（by ELISA）. The death status after operation was recorded at 3 h,〉3 6 h,〉6 24 h and 〉24-48 h. Results Compared with Sham operation group,WBC count and serum vWF concentration in sepsis and simvastatin groups were significantly increased at each time point. The peak values reached at 6 h after operation,WBC count was （7.45±0.69）× 10^9/L, （12.79±0.51） × 10^9/L and （10.36±0.19） × 10^9/ L ; vWF concentration was （0.543±0.001） ug/L, （1.198±0.035） ug/L and （1.018±0.005） ug/L（P 〈0.05）; Compared with sepsis group, WBC count and serum vWF concentration in simvastatin group were significantly decreased. The mortality decreased at 24 48 h after CLP（sepsis 75%, simvastatin 50%） （ P 〈0.05）. Conclusion Simvastatin could protect endothelial cell function,thus decrease the mortality within 48 h in septic rats.
作者 李敏芝 赵鹤龄 田东莲 李敏 李丽敏 郑龙
机构地区 河北省人民医院重症医学科 徐水县人民医院内一科 徐水县人民医院内三科 徐水县人民医院麻醉科 石家庄市疾病预防控制中心理化科 河北医科大学实验动物中心
出处 《临床荟萃》 CAS 2012年第15期1322-1324,共3页 Clinical Focus
关键词 脓毒症 动物试验 von WILLEBRAND因子 辛伐他汀 sepsis animal experimeritation von Willebrand factor simvastatin
分类号 R453.9 [医药卫生—治疗学]
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参考文献13

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共引文献22

同被引文献31

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引证文献2

二级引证文献6

临床荟萃
2012年 第15期

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