苏林酸诱导结肠腺癌HT-29细胞凋亡的实验研究

Sulindac Induced Apoptosis of HT-29 Colon Adenocarcinoma Cell

为观察苏林酸对结肠腺癌HT－29细胞有无促凋亡作用，采用流式细胞仪检测、DNA电泳和透射电镜观察苏林酸对HT－29细胞的促凋亡作用。结果：流式细胞术、DNA电泳和透射电镜观察均显示苏林酸可诱导HT－29细胞凋亡，且具有时间和剂量依赖性。用0．3mmol／L、0．6mmol／L和1．2mmol／L苏林酸处理48小时，凋亡细胞分别占5．8％、7．6％和11．7％，处理72小时则分别升为12．5％、15.4％和24．2％，而对照组凋亡细胞分别占2．9％和5．1％，处理组和对照级差异显著（P＜0．05）。以上结果表明，本实验条件下，苏林酸可诱导HT－29细胞凋亡，抑制其增殖。

This study was intended to examine whether sulindac induces apoptosis in the HT-29 cells. Flow cytometry, transmission electron microscopy and DNA electrophoresis were used. Flow cytometry, transmission electron microscopy and DNA electrophoresis all demonstrated that sulindac could induce apoptosis of the HT-29 cell line in a time- and dose-dependent manner. After treatment with 0. 3mmol/L, 0. 6mmol/L and 1. 2mmol/L sulindac for 48 hours, apoptotic cells in the treatment groups reached 5.8%, 7. 6 % and 11. 7 % versus 2. 9% in the control group; after treatment for 72 hours, apoptotic cells in the treatment groups increased to 12. 5%, 15. 4%and 24. 4% versus 5. 1 % in the control group, respectively, P<0.05.This study has demonstrated that colon adenocarcinoma HT-29 cell line can be induced by sulindac.