系统性红斑狼疮患者骨髓造血干/祖细胞体外培养及其发病机制的研究

In vitro SLE hematopoietic stem/progenitor cell growth of bone marrow and research of the pathogenesis

目的 比较正常人与系统性红班狼疮 (SLE)患者的造血干 /祖细胞生长情况及从造血干 /祖细胞水平探讨SLE的发病机制。方法 用半固体甲基纤维素集落培养法观察 16例活动期及其中 3例经治疗缓解的SLE患者的粒 巨噬细胞集落 (GM CFU)、红系集落 (E CFU)、巨核细胞集落 (MK CFU)。结果 活动期SLE患者的GM CFU、E CFU、MK CFU分别为 (182± 5 0 )、(5 6± 2 7)、(8± 5 ) ,均明显低于正常对照组 ,缓解期SLE患者的GM CFU、E CFU比活动期的明显增加 ,而MK CFU与活动期比较无明显变化。结论 活动期SLE患者的造血干 /祖细胞增生、分化功能异常 ,缓解后的SLE患者其巨核细胞系恢复迟于粒系、红系 ,SLE的发病可能与造血干

Objective To compare SLE patients′ with controls′ hematopoietic stem/progenitor cell growth of bone marrow in vitro and research on the pathogenesis of hematopoietic stem/progenitor cell.Method GM CFU,E CFU and MK CFU of 16 active SLE patients and 3 remission patients were cultured in vitro with semisolid methylcellulose.Results GM CFU,E CFU and MK CFU in active SLE were (182±50),(56±27) and (8±5),respectively,and their yield was lower than that in controls.The yield of GM CFU and E CFU in remission patients was higher than in active patients,but MK CFU had no obvious change in two groups. Conclusion Hematopoietic stem/progenitor cells of active SLE are abnormal in the functions of proliferation and differentiation.Recovery of MK CFU is later than that of GM CFU and E CFU.The abnormality of hematopoietic stem/progenitor cells may be relative to pathogenesis of SLE.