大豆体细胞胚胎发生再生体系的建立与优化

Establishment and Optimization of Regeneration System from Somatic Embryogenesis in Soybean

以耐干旱、增产潜力大、抗病毒病、品质优良的晋豆19号大豆为材料,以子叶节、下胚轴、上胚轴、子叶、真叶为外植体,研究外植体类型、激素配比、接种方式等对再生频率的影响。结果显示:无菌苗刚转绿的下胚轴是诱导愈伤组织的最适外植体;诱导愈伤的最适培养基为MS+0.2 mg/L 6-BA+1.0 mg/L 2,4-D;诱导芽分化的最适培养基为MS+1.0 mg/L 6-BA+0.05 mg/L NAA;诱导根分化的最适培养基为MS+0.25 mg/L NAA。各种外植体中,诱导频率高低依次为下胚轴、上胚轴、子叶节、子叶、真叶。

To establish a high frequency regeneration system for the gene transformation of soybean（Glycine max）,different explants from Jindou No.19 were studied and the effect of different concentration of hormone and inoculating ways was also investigated in this study.The results indicated that the hypocotyl from Jindou No.19 was more suitable for callus induction of explant in soybeans and the optimal inductive medium was MS＋0.2 mg/L 6-BA＋1.0 mg/L 2,4-D.The optimal inductive mediums for shooting and rooting were MS＋1.0 mg/L 6-BA＋0.05 mg/L NAA and MS＋0.25 mg/L NAA,respectively.In comparison with the different explants regeneration system,the regeneration frequency was as follows：hypocotyl,epicotyl,cotyledonary node,cotyledon,leaf.