摘要
由于TYLCV的宿主广泛,本试验对山东寿光地区的感病毒病辣椒进行了TYLCV感染的分子鉴定,发现寿光地区的辣椒感染了TYLCV病毒,且感染率较高。通过对病毒基因组DNA-A序列的克隆和序列分析发现,寿光感病辣椒的TYLCV序列与临沂和德州的TYLCV亲缘关系最近,并对具体突变点进行了分析。进化树分析显示,克隆得到的辣椒病毒与墨西哥的TYLCV病毒同源性较高,而与危害辣椒较严重的TYLCSV的遗传距离较远。
Molecular identification were done on tomato yellow leaf curl virus（TYLCV）isolated from pepper plants showing TYLCV symptoms in Shouguang region because TYLCV have more hosts.The result showed peppers in Shouguang infected TYLCV with high efficiency among detected plants.The isolate was the most close to the TYLCV Linyi isolate and Dezhou isolate,and the detail point mutation of Shouguang TYLCV genome compared with Linyi were analyzed.Phylogenetic tree analysis indicated that TYLCV of mexico were found to show high sequence identity with that we cloned,and with relatively low similarity to TYLCSV strains which damaged pepper seriously.
出处
《华北农学报》
CSCD
北大核心
2012年第3期46-49,共4页
Acta Agriculturae Boreali-Sinica
基金
山东省星火计划项目(2010XH0620)
潍坊学院SRTP项目
