MC4R与Leptin、MC3R和CCKAR基因的互作研究

The Interaction of MC4Rwith Leptin,MC3Rand CCKARGenes

为了研究4个与采食量相关功能基因可能存在的互作关系,采用基因重组的方法构建了黑素皮质激素受体-4(MC4R)基因的真核表达载体,采用脂质体法将该表达载体转染入成纤维细胞,瞬时转染24 h后,采用Real-timePCR的方法检测和分析了Leptin、黑素皮质激素受体-3(MC3R)和猪缩胆囊素受体(CCKAR)基因mRNA的表达变化情况。结果表明,在成纤维细胞中,过表达MC4R基因后,Leptin、MC3R和CCKAR基因的mRNA水平均无显著变化。据此推测,MC4R基因与其他3个基因虽都参与调节动物的采食量,但很可能是通过不同的调控路径来发挥功能的。

To study the potential relationships among the four genes associated with intake,recombinant method was used to build a Melanocortin receptor-4（MC4R） gene eukaryotic expression vector.The expression vector was transfected into fibroblasts by liposome method.Transient transfection after 24 h,Real-time PCR method was used to detect and analyze Leptin,MC3R and CCKAR mRNA expression changes.The results showed that,in fibroblasts,overexpression of MC4R gene,Leptin,MC3R and CCKAR mRNA levels were not significantly changed.Presumed that although these four genes are all involved in the regulation of animal feed intake,but they are likely to be regulated by a different pathway.