摘要
采用测序和PCR-RFLP的方法,分析了MSTN基因启动子区在7个品种绵羊中的单核苷酸多态性,结果表明:存在2个SNP位点(-959T/C,-784G/A),表现为6种基因型(TT、TC、CC、GG、GA、AA),在-959T/C位点中,国外肉用绵羊品种陶赛特、德国肉用美利奴及我区的巴士拜羊,TT为优势基因型,地方品种绵羊巴音布鲁克羊、多浪羊和阿勒泰羊在该位点中CC为优势基因型,经χ2检验,其群体的基因频率和基因型频率均处于Hardy-Weinberg平衡状态,群体遗传多态性分析表明,这6个绵羊品种中群体的多态信息含量(PIC)均处于0.25～0.50之间,为中度多态,特克赛尔羊在该位点未发生突变。在-784G/A位点中,除陶赛特与特克赛尔羊在该位点未发生突变,其他5个品种绵羊优势等位基因均为G等位基因。经χ2检验后,其群体的基因频率和基因型频率均处于Hardy-Weinberg平衡状态,群体遗传多态性分析表明,多浪羊、巴士拜羊和巴音布鲁克羊的群体多态信息含量(PIC)均处于0.25～0.50之间,为中度多态,而阿勒泰羊和德国肉用美利奴的群体多态信息含量(PIC)小于0.25,为低度多态。
Sequencing along with PCR-RFLP was applied to analyze the polymorphism of MSTN gene promoter in seven sheep breeds.Two SNPs at-959（T/C）and-784（G/A） including six genotype（TT,TC,CC,GG,GA,AA）respectively were detected in the promoter region,At-959（T/C）,the predominant genotype of TT was identified in Dorset,German Merino and Bashibai sheep,the CC in Bayinbuluke,Duolang and Aletai sheep.A chi-square analysis suggested that the allele frequencies and genotype frequencies of two polymorphisms were in Hardy-Weinberg equilibrium.Statistical analysis also revealed the variation of intermediate polymorphism information content（PIC） in six breeds（0.25〈PIC〈0.50）.At-784（G/A）,The G was predominant allele in all breeds,whereas the low polymorphism information content（PIC） was observed in German Meilin and Aletai populations（PIC〈0.25） and intermediate PIC in the Bayinbuluke,Duolang and Bashibai populations（0.25〈PIC〈0.50）.
出处
《华北农学报》
CSCD
北大核心
2012年第3期81-85,共5页
Acta Agriculturae Boreali-Sinica
基金
新疆维吾尔自治区国际科技合作项目(200741117)
