巴美肉羊INHA和INHBA基因多态性与产羔数关系研究

Association of INHA and INHBAGenes Polymorphism with Prolificacy of Bamei Mutton Sheep

为了研究INHA和INHBA这两个基因对巴美肉羊产羔数的影响,采用PCR-SSCP技术检测抑制素α亚基(α-inhibin,INHA)和βA亚基(βA-inhibin,INHBA)基因在巴美肉羊中的单核苷酸多态性。结果发现,引物1,4,6扩增片段有多态性,其余3对引物的扩增片段均不存在多态性。引物1,巴美肉羊有2种基因型AB型和AA型,平均产羔数AB型比AA型多0.17只(P<0.01)。引物4,巴美肉羊存在3种基因型(CC、DD和CD),巴美肉羊平均产羔数DD型比CC型多0.44只(P<0.01)。引物6,巴美肉羊有3种基因型(EE、FF和EF),EE型比EF型平均产羔数多0.21只(P<0.01),结果表明,抑制素α亚基(α-inhibin,INHA)和βA亚基(βA-inhibin,INHBA)基因是影响巴美肉羊产羔数的重要基因。

Single nucleotide polymorphism of the α-inhibin（INHA） and βA-inhibin（INHBA） genes were detected in Bamei mutton sheep by PCR-SSCP.The results indicated that there were polymorphism in the amplified region for three primer pairs（1,4 and 6）,no polymorphism in the amplified region for other three primer pairs.For primer 1,two genotypes（AA and AB） were detected in Bamei mutton sheep.The ewes with mutation heterozygous genotype AB had 0.17（P〈0.01） lambs more than those with Mutation homozygous genotype AA in Bamei mutton sheep.For primer 4,three genotypes（AA,AB and BB） were detected in Bamei mutton sheep.Sequencing revealed a mutation（G→A）at 857 bp of Exon 2 of the INHBA gene in genotype DD compared with genotype CC.This mutation did not result amino acid change.The ewes with mutation heterozygous genotype DD had 0.44（P〈0.01） lambs more than those with Mutation homozygous genotype CC.For primer 6,three genotypes（EE,FF and EF） were detected.Sequencing revealed a mutation（A→T）at 288 bp of Exon 1 of the INHA gene in genotype FF compared with genotype EE.This mutation resulted amino acid change of Met→Lys.The ewes with mutation heterozygous genotype EE had 0.21（P〈0.01） lambs more than those with Mutation homozygous genotype FF.These results preliminarily show that the INHA and INHBA genes were important genes that influence the prolificacy in Bamei mutton sheep.