透骨草提取物诱导人宫颈癌Hela细胞凋亡及其相关机制研究

The study on apoptosis of human cervical cancer Hela cell induced by Phryma Leptostachya L. var. asiatica Hara extract and related molecular mechanism

目的:探讨透骨草提取物诱导人宫颈癌Hela细胞凋亡及其相关机制。方法:荧光显微镜观察Hela细胞形态,透射电镜观察Hela细胞超微结构,流式细胞术检测Hela细胞凋亡率,Western blot检测Bcl-2蛋白和Caspase-3蛋白表达水平。结果:100μg/ml透骨草提取物处理的Hela细胞后,荧光显微镜显示Hela细胞皱缩,细胞核浓缩呈新月状边集。透射电镜可见Hella细胞表面突起和微绒毛减少,核断裂、染色质凝聚且边缘化,有"出芽"现象。流式细胞术显示透骨草提取物处理的Hela细胞凋亡率为(25.90±1.13)%,明显高于对照组(P<0.01)。Western blot结果显示透骨草提取物处理的Hela细胞Bcl-2蛋白和Caspase-3蛋白表达达明显低于与对照组(P<0.05)。结论:透骨草提取物可诱导Hela细胞凋亡,其机制可能与下调Bcl-2蛋白和Caspase-3蛋白表达有关。

Objective：To investigate the apoptosis of human cervical cancer Hela cell induced by Phryma Leptostachya L. var. asiatica Hara extract and related molecular mechanism. Methods ：The morphology of Hela cells Was observed with fluorescence microscope. The uhrastructure of Hela cells was observed with transmission electronic microscope. Flow cytometry was used to detect the ap- optosis rate of Hela cells. Western blot was used to determine the expressions of Caspase-3 and Bcl-2 proteins of Hela cells. Results ： Mter Hela cells were treated with 100 ug/ml Phryma Leptostachya L. var. asiatica Hara Extract, fluorescence microscope showed that the Hela cell shrinkage, nucleus concentrate beside theca like crescent. The transmission electronic microscope showed that the surface processes and microvillus decreased the Hela cells, nucleus fragmented, chromatin condensated and marginalized, ＂budding phenomenon＂ appeared. The flow cytometry indicated that the apoptotic rate of Hela cell was （25. 9 ± 1.13）% and increased obviously than control group（P 〈0.01 ）. The Western blot showed that the expression of Caspase-3 and Bcl-2 proteins of Hela cells decreased than control group （ P 〈 0.05 ）. Conclusion： Phryma Leptostachya L. vat. asiatica Hara extract could induce the apoptosis of Hela cells and the mechanism related to downregulating the expression of Bcl-2 and Caspase-3 protein.