宫颈癌cyclin G1表达与临床病理因素关系的研究

Expression of cyclin G1 in cervical carcinoma and its relationship with the pathologic characters

目的:探讨细胞周期蛋白G1(cyclin G1)在宫颈上皮内瘤变(CIN)和宫颈癌的表达及其与临床病理因素的关系。方法:收集石蜡包埋标本及其相关临床病理资料107例,其中正常宫颈上皮(正常对照)组18例、CIN组43例和宫颈癌组46例,用原位杂交技术检测其cyclin G1 mRNA的表达,用免疫组化二步法检测其cyclin G1蛋白的表达。结果:CIN组与宫颈癌组的cyclin G1 mRNA阳性表达率分别为46.5%和60.9%,两者差异无统计学意义(P>0.05),但均显著高于正常对照组(11.1%,P<0.05);正常对照组、CIN组和宫颈癌组的cyclin G1蛋白阳性表达率分别为5.6%、51.2%和71.7%,呈递增趋势,且差异均有显著性意义(P<0.05);在正常对照组、CINⅠ组、CINⅡ/Ⅲ组和宫颈癌组中,cyclin G1 mRNA和蛋白的阳性表达强度与宫颈病变级别均呈正相关(r分别为0.378和0.487,P<0.05);cyclin G1蛋白与cyclin G1 mRNA的表达呈显著正相关(r=0.530,P=0.000);宫颈癌组cyclin G1 mRNA的表达与各项临床病理因素均无相关性(P>0.05),但cyclin G1蛋白的表达与淋巴结转移有关(P<0.05)。结论:cyclin G1 mRNA和蛋白在宫颈癌中均有高表达,可能与宫颈癌的发生有关;cyclinG1蛋白的高表达可能是其mRNA转录水平增高所致,并且可能与宫颈癌恶性侵袭有关。

Objective：To study the expression of eyclin G1 and its correlation with the pathologic characters in patients with normal cervical tissue, CIN as well as cervical carcinoma. Methods： In situ hybridization was used to detect the expression of cyclin GI mRNA and immunohistochemistry was used to detect the expression of cyclin G1 protein in the same patients ,which include 18 cases of normal cervix tissue ,43 cases of CIN and 46 cases of cervical carcinoma. The potential correlation between cyclin GI mRNA and cyclin G1 protein was also studied. Results ： The positive expression rates of cyclin G1 mRNA in CIN （46.5%） and cervical carcinoma （60.9 % ） were significantly higher than normal（ 11.1%, P 〈 0.05 ）. The positive expression rates of cyclin G1 protein , which in norreal cervical tissue（ 5.6% ）, CIN （51.2%） and cervical carcinoma（71.7% ）, were gradually increasing. The differences among them were statistically significant,P 〈 0.05. The expression of cyclin G1 protein had positive correlation with the expression of cyclin G1 mRNA. In cervical carcinoma, the expression of cyclin G1 mRNA had no relationships with all pathologic characters （P 〉 0.05 ） , while the expression of cyclin G1 protein had significant correlation with lymph-node metastasis （P 〈 0.05 ）. Conclusion ： The overexpression of cyclin G1 mRNA and protein might play an important role in human cervical tumorigenesis. The overexpression of cyclin G1 protein might because of increased cyclin G1 mRNA level, and might be related to the metastasis of cervical carcinoma.