顺铂对肝癌细胞株HepG2增殖和SATB1表达的影响

Effects of cisplatin on the proliferation and SATB1 expression of HepG2 cells

目的:观察特异AT序列结合蛋白1(special AT-rich sequence-binding protein 1,SATB1)在不同肝癌细胞株中的表达情况,并探讨顺铂对肝癌细胞株HepG2的细胞形态及SATB1表达的影响。方法:半定量反转录聚合酶链反应(RT-PCR)检测HepG2、BEL-7402、SMMC-7721三种肝癌细胞株中SATB1 mRNA的表达情况;HepG2细胞株中加入终浓度为2.5、5.0和10.0μg/ml顺铂培养24 h,倒置显微镜下观察细胞形态变化。结果:SATB1在肝癌细胞株BEL-7402、SMMC-7721、HepG2中均有表达,差异无统计学意义(P>0.05)。HepG2细胞与顺铂共培养24 h后,倒置显微镜下可见细胞形态明显改变,细胞数减少,损伤、死亡的细胞增多。SATB1 mRNA的表达随着顺铂浓度的增加而减少,其中5.0、10.0μg/ml顺铂组SATB1 mRNA的表达量与对照组差异均有统计学意义(P<0.05)。结论:SATB1在三种肝癌细胞株中均有表达,顺铂可抑制HepG2细胞增殖和SATB1的表达,从而达到抑制肝癌细胞生长的目的。

Objective：To observe the expressions of the special AT-rich sequence-binding protein 1 （SATB1） in different hepatoma cell lines and the effects of Cisplatin on the morphology and SATB1 expression of HepG2 cells. Methods： The mRNA expressions of SATB1 of three hepatoma cell lines, HepG2, BEL-7402 and SMMC-7721 were detected using reverse transcription polymerase chain reaction. After HepG2 cells were treated with different concentrations （2.5,5.0 and 10.0μg/ml） of Cisplatin for 24 hours, the cell morphology were observed with inverted microscope and the mRNA expressions of SATB1 were examined using RT-PCR. Results：The expressions of SATB1 in BEL-7402, SMMC-7721 and HepG2 had no statistically significant difference（ P 〉 0.05 ）. The changes of cell morphology, the decreasing in cell number and increasing in cell damage and death were observed under inverted microscope after HepG2 cells were treated with Cisplatin for 24 hours. The mRNA expressions of SATB1 decreased gradually with the increasing concentrations of Cisplatin, and the mRNA expressions of SATB1 treated with 5. 0 and 10. 0μg/ml of Cisplatin had statistical significance compared with that of control group（ P 〈0.05）. Conclusions： All three hepatoma cell lines can express SATB1. Cisplatin can inhibit the proliferation of HepG2 cells and the expression of SATB1, so as to inhibit the growth of hepatoma cells.