摘要
采用高效液相色谱方法,考察了流动相和流速对果糖嗪定性分析结果的影响.结果表明在确定Waters Carbohydrate Analysis为分离柱,检测波长为275 nm,进样量为10μL条件下,流动相为乙腈与水,其体积比为80∶20,流速为1.0 mL/min时,定性分析效果最佳.
The effect of mobile phase and flow rate on the qualitative analysis of fructosazine with high per- formance liquid chromatography was investigated. The results showed that the effect of qualitative analysis was the best using acetonitrile and water as mobile phase （ 80： 20, V/V） and flow rate 1.0 mL/min under condition as follows：Water Carbohydrate Analysis works as separation column, detection wavelength is 275 nm,and the volume of injection is 10μL.
出处
《郑州轻工业学院学报（自然科学版）》
CAS
2012年第4期65-68,共4页
Journal of Zhengzhou University of Light Industry:Natural Science
基金
河南省科技厅科技攻关(重点项目)(102102210135)
关键词
果糖嗪
高效液相色谱分析
流动相
流速
fructosazine
high performance liquid chromatography analysis
mobile phase
flow rate
