不良孕产夫妇核仁组织区rRNA基因活性的研究

Study on Activity of NOR rRNA Gene in Couples With Adverse Pregnancy

目的 :研究不良孕产夫妇核仁组织区rRNA基因活性的特点。方法 :应用外周血淋巴细胞染色体常规制备方法和Ag -NOR银染技术 ,分析 5 2对不良孕产夫妇及 5 6对正常夫妇染色体标本 ,计数银染NOR数目。结果 :不良孕产夫妇和对照组每个细胞银染NOR均数分别为 7 2 15 0± 0 5 438、6 85 96± 0 5 96 5。统计学分析表明 ,两者有明显差异 (P <0 0 5 )。结论 :不良孕产夫妇NORrRNA基因活性明显增高。NORrRNA基因活性增高可能通过其蛋白产物增高随体联合频率和覆盖着丝粒———动粒复合体而影响染色体的正常分离 。

Objective: To search the regular pattern of NOR (nuclear organizer region) rRNA gene activity in couples with adverse pregnancy. Methods: Ag-NOR numbers of couples with adverse pregnancy and of controls were analysed by using the chromosomal Ag-NOR technique. Results: The mean of Ag-NOR number per cell of the two groups was 7 2150±0 5438、6 8596±0 5965 respectively, the difference between them was statistically significant P<0 05. Conclusion: The higher activity of NOR rRNA gene may be the main reason for causing adverse pregnancy by increasing the frequency of SA and influencing the function of centromere kinetochore complex.