糖尿病人血浆肿瘤坏死因子α初步测定

PRELIMINARY DETERMINATIONS OF PLASMA TUMOR NECROSIS FACTOR-α LEVEL IN DIABETES MELLITUS

目的 :探讨两型糖尿病人血浆肿瘤坏死因子 - α(TNF- α)水平的变化。方法 :选取正常对照 (正常组 )、 1型糖尿病(DM1组 )和 2型糖尿病 (DM2组 )共 3组研究对象 ,再将 2型糖尿病组按体质量指数 (BMI) <2 5的编为 DM2 a组 ,≥ 2 5的编为 DM2 b组 ;用酶联免疫法 (EL ISA)测定各组的 TNF- α水平并比较。结果 :正常对照组血浆 TNF- α为 (0 .90±0 .0 7) μg/L,1型糖尿病组为 (0 .86± 0 .0 6 ) μg/L,与正常组比较无差异 (P >0 .0 5 ) ;2型糖尿病组中 BMI<2 5者为 (1.0 2± 0 .2 8) μg/L,比正常组升高 (P <0 .0 5 ) ,BMI≥ 2 5者为 (2 .2 3± 0 .41) μg/L,与正常组比显著升高 (P <0 .0 1) ,与 BMI<2 5者比 ,两者差异亦有显著意义 (P <0 .0 1)。结论 :2型糖尿病人血浆 TNF- α水平显著高于正常人和 1型糖尿病人 ,并与肥胖有一定的关系。

Objective:To study the level change of plasma tumor necrosis factor α (TNF α) in type 1 diabetes and type 2 diabetes Methods:The plasma concentrations of TNF α were determined by ELISA in 12 control subjects (Control group),10 type 1 diabetes (DM1 group) and 19 type 2 diabetes (DM2 group),which were further subdivided into 2 subgroups based on BMI as DM2a group (BMI<25) and DM2b group (BMI≥25).Results:The plasma TNF α level showed no sigificant difference (P>0 05) between DM1 and Control group;the TNF α level in DM2a and DM2b all increased significantly as compared with Control group (P<0 05 and 0 01),and the TNF α level in DM2b was significantly higher than that in DM2a (P<0 01).Conclusion:The concentration of TNF α increased significantly in type 2 diabetes as compared to normal and type 1 diabetes subjects,and it was correlative with obesity