COPD患者诱导痰中IL-10、TNF-α、8-iso-PGF2α水平与吸烟及肺功能的关系

Relation among the levels of IL-10,TNF-α,8-iso-PGF2α in induced sputum and lung function and smoking in patients with chronic obstructive pulmonary disease

目的探讨慢性阻塞性肺疾病(COPD)稳定期患者诱导痰中白细胞介素10(IL-10)、肿瘤坏死因子(TNF-α)、8-异前列腺素F2α(8-iso-PGF2α)的水平与吸烟及肺功能的关系。方法选择COPD急性发作频率≥3次/年的患者15例(A组)、COPD急性发作频率≤2次/年的患者15例(B组)、健康吸烟者12例(C组)、健康非吸烟者10例(D组)。分别测定4组诱导痰中IL-10、TNF-α、8-iso-PGF2α水平和肺功能指标。结果①A组TNF-α、8-iso-PGF2α水平高于B组,但无统计学差异(P>0.05);显著高于C、D组(P<0.05)。②A组IL-10水平显著低于B、C、D组(P<0.05)。③TNF-α、8-iso-PGF2α水平与第1秒用力呼气容积占预计值百分比(FEV1%)、1秒率(FEV1/FVC)呈负相关(r分别为-0.742、-0.639,P均<0.05);IL-10水平与FEV1%、FEV1/FVC呈正相关(r分别为0.476、0.537,P均<0.05)。结论吸烟可使TNF-α、8-iso-PGF2α水平升高,IL-10水平下降;IL-10、TNF-α、8-iso-PGF2α对患者肺功能有影响,它们参与了COPD气道慢性炎症的过程,对气道重塑可能起重要作用。

Objective To investigate the rlation among the levels of interlenkin-10 （IL-10）, tumor necrosis factor （TNF-ot）, prostaglandin （8-iso-PGF2α） in induced sputum and lung function and somoking in patients with stable chronic obstructive pulmonary disease （COPD）. Methods Fifteen patients with stable COPD （incidence equal to or greater than third a year, A group） and 15 patients with stable COPD （incidence equal to or less than twice a year, B group） and 12 smokers （C group） and 10 healthy control subjects （D group） were enrolled in the study. Levels of IL-10, TNF-α, 8-iso- PGF2α in sputum supernatant were measured by ELISA and lung function were detected. Results The levels of TNF-α, 8-iso-PGF2α in group A were significantly higher than those in group C and D （ all P 〈 0.05）, and were higher than those in group B （all P 〉0.05）. The level of IL-10 in group A was significantly lower than that in group group B, C and D （P 〈 0.05）. The levels of TNF-α, 8-iso-PGF2α were inversely correlated with FEV~% and FEV2/FVC （r = -0. 742, -0. 639,both P 〈0.05）. The level of IL-10 was positively correlated with FEV1% and FEV~/FVC （r =0. 476, 0.537,both P 〈0.05）. It was founded that levels of TNF-c~, 8-iso-PGF2oL were increased and the level of IL-10 was decreased by smoking. Conclusions The levels of IL-10, TNF-α, 8-iso-PGF2α in the patients with COPD have influence on pulmonary function. It may be involved in the airway inflammatory process and contribute to airway remodeling in COPD.