银杏叶提取物抑制大鼠急性肺损伤时ICAM-1蛋白的表达

Inhibitory Effects of Ginkgo Biloba Extract on ICAM-1 Expression of Rats with Acute Lung Injury

目的:探讨银杏叶提取物(ginkgo biloba extract,GBE)对大鼠急性肺损伤(acute lung injury,ALI)时肺脏的保护作用及其可能机制。方法:随机将大鼠分为4组:对照组,脂多糖(lipopolysaccharide,LPS)组,地塞米松(dexamethasone,DEX)组和GBE处理组。各组动物分别于尾静脉注射试剂后4、8和16 h检测肺组织超氧化物歧化酶(superoxide dismutase,SOD)活性及丙二醛(malonaldehyde,MDA)含量,也借助免疫组化方法检测细胞间黏附分子-1(ICAM-1)在肺组织的表达。结果:LPS组SOD活性较对照组明显降低(P<0.01),而MDA含量及ICAM-1的表达则显著升高(P<0.01);应用GBE可显著缓解上述变化(P<0.05)。结论:GBE可能通过抑制ICAM-1的表达而缓解ALI时肺脏的氧化应激损伤。

Objective：To investigate the protective effect of ginkgo biloba extract （GBE） on lung tissues during acute lung injury （ALI） in rats and its possible mechanism. Methods：all rats were randomly divided into four groups： Control group, Lipopolysaccharide（LPS）group, dexamethasone （DEX） and GBE treatment group. Superoxide dismutase （SOD） activity and malonaldehyde （MDA） content Of lung tissues were detected at 4,8 and 16 h after tail intravenous in each groups. In addition, the expression of ICAM- 1 was observed via im- munohistochemistry staining in lung tissues. Results：Compared with Control group, SOD activity decreased sig- nificantly in LPS group（P ~ 0.01）, but MDA content and the expression of ICAM- 1 increased obviously（P 〈 0.01）; the administration of GBE or DEX mitigated above changes significantly（ P 〈 0.05）. Conclusion： GBE possessed protective effect on lung tissues during ALI through scavenging free radicals and inhibiting the expression of ICAM- 1.