西安地区汉族健康人群GPx-1基因Pro 198Leu多态性分析

Analysis of GPx-1 Genes Pro198Leu Polymorphism in Xi＇an Han Healthy Population

目的了解西安地区汉族健康人群GPx-1基因单核苷酸多态性位点分布特点。方法抽取西安地区汉族健康人420例，采用PCR-RFLP方法，检测GPx-1基因第2外显子上的Pro198Leu基因多态性分布。结果GPx-1基因第2外显子上Pro198Leu有三种等位基因型，C／C（Pro198Leu），C／T（Pro198Leu）和T／T（Leu198Leu），分别占92．4％，6．9％和0．7％。计算Hardy-Weinberg平衡，结果χ^2=3．387，P〉0．05，符合Hardy-Weinberg平衡，具有群体代表性。结论西安地区汉族健康人群GPx-1基因Pro198Leu多态基因型以CC型为主，频率分布与文献报道中的日本人和韩国人相似，与美国人和芬兰人相差显著。

Objective To understand the distribution of Xi＇an Han healthy population GPx,-1 gene single nucleotide polymorphisms （SNPs）. Methods Extracted 420 of Xi＇an in Chinese Han healthy people,using PCR-RFLP method to detect the Pro198Leu gene polymorphism in the exon second of GPx-1 gene. Results On the Pro198Leu GPx-1 gene exon three alleles, the C/C （Pro 198Leu）, the C/T （Pro 198Leu） and T/T （Leu198Leu）, respectively, accounted for 92.4 G, 6.9 % and 0.7 %,respectively. Calculated the Hardy-Weinberg equilibrium, χ^2 = 3. 387, P〉 0. 05, the Hardy-Weinberg equilibrium, with a group representative. Conclusion Xi＇an Han population GPx 1 gene Pro198Leu polymorphism genotype CC genotypebased, similar to the frequency distribution reported in the literature in Japanese and Korean, significant difference between the Americans and Finns.