血清和肽素ELISA定量测定改良法的建立和应用

Establishing Improvement of Enzyme-Linked Immunosorbent Assay for the Quantitative Determination of Copeptin in Serum and Its Application

目的建立血清和肽素ELISA法定量测定的改良法，并探讨其应用价值。方法用定量ELISA法测定患者和健康者的血清和肽素。结果该法检出限为0．018pmol／L，线性为0．075～24．0pmol／L，平均回收率为98．8％，批内CV为3．39％，批间CV为5．58％，参考区间为2．1～12．3pmol／L。21例脓毒症和31例获得性肺炎及33例急性心肌梗死与52例心力衰竭患者的血清和肽素值分别为48．96±5．80，32．82±5．02，68．51±5．63和23．14±5．47pmol／L，它们之间的差异具有统计学意义（F=501．542，P=0．000）。结论该法灵敏、特异和准确，血清和肽素定量测定是诊断脓毒症、获得性肺炎、急性心肌梗死和心力衰竭的较好实验指标。

Objective To establish enzyme-linked immuneosorbent assay （ELISA） of improvement for the quantitative determination of a C-terminal portion of pro-vasopressin（copeptin） in serum and to investigate its clinical application. Methods Serum copeptin of patients and of normal controls were measured by ELISA. Results The limit of detection （LOD） was 0. 018 pmol/L. The linearity was 0. 075 - 24. 0 pmol/L. The average rate of recovery was 98.8%. Within-run CV was 3.39 % and between-run CV was 5.58 %. The reference interval were 2. 1 -1 2. 3 pmol/L. The serum copeptin concentrations of 21 patients with sepsis were 48. 96±5.80 pmol/L,of 31 patients with acquired pneumonia were 32. 82±5. 02 pmol/L of 33 patients with acute myocardial infarction were 68.51± 5.63 pmol/L and of 52 patients with chronic heart failure were 23.14±5.47 pmol/L. Significant differences were observed in them （F=501. 542, P=0. 000）. Conclusion This method is sensitive,specific and accurate. Quantitative detection of eopeptin is now better laboratory test for patients of sepsis of ac- quired pneumonia of acute myocardial infarction and of Chronic heart failure.