48份甜玉米自交系的遗传多样性分析

Genetic diversity analysis of 48 sweet corn inbred lines based on SSR markers

以48份甜玉米自交系为材料,采用40个SSR标记检测其基因组DNA,探究其等位变异情况和自交系间的亲缘关系。结果表明,共检测到86个位点的等位基因变异,平均每对引物检测出的等位基因变异为2.15个,变异范围为1～4个,平均多态性信息含量为0.49,变异范围为0.04～0.80。聚类结果将48个甜玉米自交系分为3个类群,与材料来源基本吻合。

The DNA of 48 sweet corn inbred lines was determined by 40 SSR markers to study allelic variation of these SSR markers loci and the relationship between 48 tested sweet corn inbred lines. A toral of 86 polymorphic bands were detected and 1 ~4 alleles for each primer, averaging with 2.15 alleles per primer. The mean content of polymorphic information was 0.49, ranging from 0.04 to 0.80. The 48 sweet corn inbred lines were devided into 3 groups, which was similar to the germplasmic source.